By car (itinerary from Italy)

> Brenner
> Innsbruck
> Monaco
> A93 direction Regensburg - Schwandorf
> Waidhaus/Rozvadov - German/Czech border
> Plzen
> Prague

You are obliged to buy a toll sticker to use highways and motorways in the Czech Republic - these are available at almost every gas station. Priced around CZK 220 (EUR 10), they are valid for 7 days.